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Learn about the causes of enteritis with a human-like mini-chip: Dong-A Science



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November 9, 2013 03:34

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Long-term chip developed by the Texas State University Research Group and Professor Kim Hyun-John. The intestinal epithelium grows to form the intestinal surface. Experiments are performed by injecting the medium and the medicine. - Photo presentation by Professor Kim Hyun Joons

Long-term chip developed by the Texas State University Research Group and Professor Kim Hyun-John. The intestinal epithelium grows to form the intestinal surface. Experiments are performed by injecting the medium and the medicine. – Photo presentation by Professor Kim Hyun Joons

Mice suffering from ulcers are ultimately mixed with faeces and blood. This is enteritis. Herbal nutrition called DSS laboratory rats can cause enteritis. I'm sorry to regret, but it was an animal experiment that I had to do to find out the cause of the disease.

It is expected that such things will disappear in the future. This is because the "long-term chip" of the experimental plant, which cultivates human cells, and not animals, and places them in pieces of plastic, employs only thumb nails each day. Now is a step to find out the cause of the disease without animals.

The main feature of this technique is the graduate who wanted to reduce the animals suffering from enteritis and a professor who developed a long-term chip simulating the field six years ago. They found the cause of enteritis by using a long-term chip that was developed in a company without an experimental animal, and published in an October issue of the journal, the National Academy of Sciences (PNAS) magazine.

The Texas University researcher Austin Shin Woo Jung and Professor Kim Hyun Joong team produced two layers of long-lasting chips with fine filters. In the upper layer, intestinal epithelial cells made from cells (cancer cells) that do not die, but continue to spread, are inserted and created to create the intestinal environment, including a villa-like structure. The epithelial cells were closely interconnected with each other, and in the lower layer isolated from the blood isolated immune cells to create an environment that mimics the blood vessels outside the intestines.

(Left) and Kim Hyun-jun, Professor at the University of Texas-Texas. - Photo presentation by Professor Kim Hyun Joons

(Left) and Kim Hyun-jun, Professor at the University of Texas-Texas. – Photo presentation by Professor Kim Hyun Joons

The researchers poured the DSS micron in the upper layer, which was completed in such a way as to cause an enteritis and confirm the onset of the disease. In an e-mail interview, Professor Kim said: "We tested with a long-term combination of chips, whether intestinal microorganisms are the cause of inflammation, whether the problem is activated immune cell attack, or the collapse of the epithelial cell barrier is a problem." The most serious study was due to damage to the epithelium cell wall barrier, which was originally set, "he said.

Initial gut epithelial cells are closely linked together to "protect the inside of the bowel and the outside" without "wet". However, as a result of the study, with the development of the enteritis, the conjunctiva develops and a hole is formed, and intestinal microorganisms enter the outer blood vessels through a hole and meet the immune cells, causing problems. In the past, it was thought that DSS destroys epithelial cells, but it also confirmed that cell destruction is not the main cause.

Practical guidelines for treatment and symptom relief were also obtained. Probiotics, which means beneficial organisms for the body, such as lactobacillus, have a good effect on the original intestine. However, a team of researchers confirmed that, by stopping the intestinal wall and receiving lactic acid bacteria, these microorganisms leaked from the intestines and became quite problematic. Also causes serious sepsis. If the bowel wall is seriously injured, this means that lactic acid bacteria should be carefully noted.

The long-term chip structure developed by the new researcher and Professor Kim's team. Based on the average filter, the epithelial cells are located on the upper part and the blood immune cells are located on the lower side. Experiments are performed by skipping a nutrient and an enteritis that induces a DSS substance through each channel. - Photo presentation by Professor Kim Hyun Joons

The long-term chip structure developed by the new researcher and Professor Kim's team. Based on the average filter, the epithelial cells are located on the upper part and the blood immune cells are located on the lower side. Experiments are performed by skipping a nutrient and an enteritis that induces a DSS substance through each channel. – Photo presentation by Professor Kim Hyun Joons

The study was the first in the world to determine the causes and processes of outbreaks through long-term chips. "We plan to develop long-term chips using separate cells and stem cells to create patient-specific chips that reflect individual genetic and environmental factors," said the researcher. To study intestinal microorganisms, we plan to grow intestinal microorganisms that are separated from the patient's stools into a long-range chip. In addition to enteritis, it can be applied to colorectal cancer or inflammatory bowel disease (IBD), a new researcher said.

The research was funded by the International Ethics Experimental Fund (IFER) and the Korean Research Foundation. IFER supports alternative studies to replace animal experiments.

November 9, 2013 03:34

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